Efficient and Rapid C. elegans Transgenesis by Bombardment and Hygromycin B Selection

We report a simple, cost-effective, scalable and efficient method for creating transgenic Caenorhabditis elegans that requires minimal hands-on time. The method combines biolistic bombardment with selection for transgenics that bear a hygromycin B resistance gene on agar plates supplemented with hyg...

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Detalles Bibliográficos
Autores principales: Radman, Inja, Greiss, Sebastian, Chin, Jason W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794024/
https://www.ncbi.nlm.nih.gov/pubmed/24130756
http://dx.doi.org/10.1371/journal.pone.0076019

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794024/
https://www.ncbi.nlm.nih.gov/pubmed/24130756
http://dx.doi.org/10.1371/journal.pone.0076019

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