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Efficient and Rapid C. elegans Transgenesis by Bombardment and Hygromycin B Selection
We report a simple, cost-effective, scalable and efficient method for creating transgenic Caenorhabditis elegans that requires minimal hands-on time. The method combines biolistic bombardment with selection for transgenics that bear a hygromycin B resistance gene on agar plates supplemented with hyg...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2013
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794024/ https://www.ncbi.nlm.nih.gov/pubmed/24130756 http://dx.doi.org/10.1371/journal.pone.0076019 |