High-Level Overproduction of Thermobifida Enzyme in Streptomyces lividans Using a Novel Expression Vector

In this study, we constructed a novel Streptomyces-E.coli shuttle vector pZRJ362 combining the xylose isomerase promoter and amylase terminator. A gene encoding the endoglucanase Cel6A in Thermobifida fusca was amplified by PCR, cloned into Streptomyces lividans host strain using the novel expressio...

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Detalles Bibliográficos
Autores principales: Li, Jun-Xia, Zhao, Long-Mei, Wu, Ru-Juan, Zheng, Zhao-Jun, Zhang, Ri-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794799/
https://www.ncbi.nlm.nih.gov/pubmed/24025422
http://dx.doi.org/10.3390/ijms140918629

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794799/
https://www.ncbi.nlm.nih.gov/pubmed/24025422
http://dx.doi.org/10.3390/ijms140918629

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