Cargando…
High-Level Overproduction of Thermobifida Enzyme in Streptomyces lividans Using a Novel Expression Vector
In this study, we constructed a novel Streptomyces-E.coli shuttle vector pZRJ362 combining the xylose isomerase promoter and amylase terminator. A gene encoding the endoglucanase Cel6A in Thermobifida fusca was amplified by PCR, cloned into Streptomyces lividans host strain using the novel expressio...
| Autores principales: | , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
MDPI
2013
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794799/ https://www.ncbi.nlm.nih.gov/pubmed/24025422 http://dx.doi.org/10.3390/ijms140918629 |