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An Improved SELEX-Seq Strategy for Characterizing DNA-Binding Specificity of Transcription Factor: NF-κB as an Example

SELEX-Seq is now the optimal high-throughput technique for characterizing DNA-binding specificities of transcription factors. In this study, we introduced an improved EMSA-based SELEX-Seq strategy with several advantages. The improvements of this strategy included: (1) using a FAM-labeled probe to t...

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Detalles Bibliográficos
Autores principales: Gu, Guangming, Wang, Tingting, Yang, Yang, Xu, Xinhui, Wang, Jinke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794954/
https://www.ncbi.nlm.nih.gov/pubmed/24130762
http://dx.doi.org/10.1371/journal.pone.0076109