Cargando…
An Improved SELEX-Seq Strategy for Characterizing DNA-Binding Specificity of Transcription Factor: NF-κB as an Example
SELEX-Seq is now the optimal high-throughput technique for characterizing DNA-binding specificities of transcription factors. In this study, we introduced an improved EMSA-based SELEX-Seq strategy with several advantages. The improvements of this strategy included: (1) using a FAM-labeled probe to t...
Autores principales: | Gu, Guangming, Wang, Tingting, Yang, Yang, Xu, Xinhui, Wang, Jinke |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794954/ https://www.ncbi.nlm.nih.gov/pubmed/24130762 http://dx.doi.org/10.1371/journal.pone.0076109 |
Ejemplares similares
-
DNA-binding properties of the MADS-domain transcription factor SEPALLATA3 and mutant variants characterized by SELEX-seq
por: Käppel, Sandra, et al.
Publicado: (2021) -
Single-Nucleotide Mutation Matrix: A New Model for Predicting the NF-κB DNA Binding Sites
por: Du, Wenxin, et al.
Publicado: (2014) -
High-throughput sequencing SELEX for the determination of DNA-binding protein specificities in vitro
por: Pantier, Raphaël, et al.
Publicado: (2022) -
Characterization of homing endonuclease binding and cleavage specificities using yeast surface display SELEX (YSD-SELEX)
por: Jacoby, Kyle, et al.
Publicado: (2017) -
HTPSELEX—a database of high-throughput SELEX libraries for transcription factor binding sites
por: Jagannathan, Vidhya, et al.
Publicado: (2006)