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Direct confocal acquisition of fluorescence from X-gal staining on thick tissue sections

X-gal staining is a common procedure used in the histochemical monitoring of gene expression by light microscopy. However, this procedure does not permit the direct confocal acquisition of images, thus preventing the identification of labelled cells on the depth (Z) axis of tissue sections and leadi...

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Detalles Bibliográficos
Autores principales: Levitsky, Konstantin L., Toledo-Aral, Juan José, López-Barneo, José, Villadiego, Javier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3796308/
https://www.ncbi.nlm.nih.gov/pubmed/24121824
http://dx.doi.org/10.1038/srep02937