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4Pi fluorescence detection and 3D particle localization with a single objective

Coherent detection through two opposing objectives (4Pi configuration) improves the precision of three-dimensional (3D) single-molecule localization substantially along the axial direction, but suffers from instrument complexity and maintenance difficulty. To address these issues, we have realized 4...

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Detalles Bibliográficos
Autores principales: Schnitzbauer, J., McGorty, R., Huang, B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3796685/
https://www.ncbi.nlm.nih.gov/pubmed/24105517
http://dx.doi.org/10.1364/OE.21.019701