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Combining Different mRNA Capture Methods to Analyze the Transcriptome: Analysis of the Xenopus laevis Transcriptome

mRNA sequencing (mRNA-seq) is a commonly used technique to survey gene expression from organisms with fully sequenced genomes. Successful mRNA-seq requires purification of mRNA away from the much more abundant ribosomal RNA, which is typically accomplished by oligo-dT selection. However, mRNAs with...

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Detalles Bibliográficos
Autores principales: Blower, Michael D., Jambhekar, Ashwini, Schwarz, Dianne S., Toombs, James A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3797054/
https://www.ncbi.nlm.nih.gov/pubmed/24143257
http://dx.doi.org/10.1371/journal.pone.0077700