Torsionally constrained DNA for single-molecule assays: an efficient, ligation-free method

Controlled twisting of individual, double-stranded DNA molecules provides a unique method to investigate the enzymes that alter DNA topology. Such twisting requires a single DNA molecule to be torsionally constrained. This constraint is achieved by anchoring the opposite ends of the DNA to two separ...

Descripción completa

Detalles Bibliográficos
Autores principales: Paik, D. Hern, Roskens, Violet A., Perkins, Thomas T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3799452/
https://www.ncbi.nlm.nih.gov/pubmed/23935118
http://dx.doi.org/10.1093/nar/gkt699

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3799452/
https://www.ncbi.nlm.nih.gov/pubmed/23935118
http://dx.doi.org/10.1093/nar/gkt699

Ejemplares similares