Cargando…

In silico abstraction of zinc finger nuclease cleavage profiles reveals an expanded landscape of off-target sites

Gene-editing nucleases enable targeted modification of DNA sequences in living cells, thereby facilitating efficient knockout and precise editing of endogenous loci. Engineered nucleases also have the potential to introduce mutations at off-target sites of action. Such unintended alterations can con...

Descripción completa

Detalles Bibliográficos
Autores principales:	Sander, Jeffry D., Ramirez, Cherie L., Linder, Samantha J., Pattanayak, Vikram, Shoresh, Noam, Ku, Manching, Foden, Jennifer A., Reyon, Deepak, Bernstein, Bradley E., Liu, David R., Joung, J. Keith
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2013
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3799455/ https://www.ncbi.nlm.nih.gov/pubmed/23945932 http://dx.doi.org/10.1093/nar/gkt716

Ejemplares similares

Revealing Off-Target Cleavage Specificities of Zinc Finger Nucleases by In Vitro Selection
por: Pattanayak, Vikram, et al.
Publicado: (2011)

Broad Specificity Profiling of TALENs Results in Engineered Nucleases With Improved DNA Cleavage Specificity
por: Guilinger, John P., et al.
Publicado: (2014)

High frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells
por: Fu, Yanfang, et al.
Publicado: (2013)

An online bioinformatics tool predicts zinc finger and TALE nuclease off-target cleavage
por: Fine, Eli J., et al.
Publicado: (2014)

ZFNGenome: A comprehensive resource for locating zinc finger nuclease target sites in model organisms
por: Reyon, Deepak, et al.
Publicado: (2011)

Rapid Mutation of Endogenous Zebrafish Genes Using Zinc Finger Nucleases Made by Oligomerized Pool ENgineering (OPEN)
por: Foley, Jonathan E., et al.
Publicado: (2009)

Targeted Deletion and Inversion of Tandemly Arrayed Genes in Arabidopsis thaliana Using Zinc Finger Nucleases
por: Qi, Yiping, et al.
Publicado: (2013)

Improving CRISPR-Cas nuclease specificity using truncated guide RNAs
por: Fu, Yanfang, et al.
Publicado: (2014)

Improved Somatic Mutagenesis in Zebrafish Using Transcription Activator-Like Effector Nucleases (TALENs)
por: Moore, Finola E., et al.
Publicado: (2012)

ZiFiT (Zinc Finger Targeter): an updated zinc finger engineering tool
por: Sander, Jeffry D., et al.
Publicado: (2010)

Efficient In Vivo Genome Editing Using RNA-Guided Nucleases
por: Hwang, Woong Y., et al.
Publicado: (2013)

FLASH Assembly of TALENs Enables High-Throughput Genome Editing
por: Reyon, Deepak, et al.
Publicado: (2012)

Selection-Free Zinc-Finger Nuclease Engineering by Context-Dependent Assembly (CoDA)
por: Sander, Jeffry D., et al.
Publicado: (2010)

Zinc-finger nuclease-driven targeted integration into mammalian genomes using donors with limited chromosomal homology
por: Orlando, Salvatore J., et al.
Publicado: (2010)

Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing
por: Tsai, Shengdar Q., et al.
Publicado: (2014)

High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity
por: Pattanayak, Vikram, et al.
Publicado: (2013)

A novel zinc-finger nuclease platform with a sequence-specific cleavage module
por: Schierling, Benno, et al.
Publicado: (2012)

Expanding the Repertoire of Target Sites for Zinc Finger Nuclease-mediated Genome Modification
por: Wilson, Kimberly A, et al.
Publicado: (2013)

High-efficiency genome editing via 2A-coupled co-expression of fluorescent proteins and zinc finger nucleases or CRISPR/Cas9 nickase pairs
por: Duda, Katarzyna, et al.
Publicado: (2014)

Engineered apoptotic nucleases for chromatin research
por: Xiao, Fei, et al.
Publicado: (2007)

Autonomous zinc-finger nuclease pairs for targeted chromosomal deletion
por: Şöllü, Cem, et al.
Publicado: (2010)

Receptor-mediated delivery of engineered nucleases for genome modification
por: Chen, Zhong, et al.
Publicado: (2013)

New approach to real-time nucleic acids detection: folding polymerase chain reaction amplicons into a secondary structure to improve cleavage of Förster resonance energy transfer probes in 5′-nuclease assays
por: Kutyavin, Igor V.
Publicado: (2010)

Robust, synergistic regulation of human gene expression using TALE activators
por: Maeder, Morgan L., et al.
Publicado: (2013)

Zinc Finger Targeter (ZiFiT): an engineered zinc finger/target site design tool
por: Sander, Jeffry D., et al.
Publicado: (2007)

Error correction of microchip synthesized genes using Surveyor nuclease
por: Saaem, Ishtiaq, et al.
Publicado: (2012)

Using defined finger–finger interfaces as units of assembly for constructing zinc-finger nucleases
por: Zhu, Cong, et al.
Publicado: (2013)

High frequency modification of plant genes using engineered zinc finger nucleases
por: Townsend, Jeffrey A., et al.
Publicado: (2009)

SAPTA: a new design tool for improving TALE nuclease activity
por: Lin, Yanni, et al.
Publicado: (2014)

A cleavage-based surrogate reporter for the evaluation of CRISPR–Cas9 cleavage efficiency
por: Jung, Soo Bin, et al.
Publicado: (2021)

Quantitative telomeric overhang determination using a double-strand specific nuclease
por: Zhao, Yong, et al.
Publicado: (2008)

Duplex-specific nuclease efficiently removes rRNA for prokaryotic RNA-seq
por: Yi, Hana, et al.
Publicado: (2011)

Predicting success of oligomerized pool engineering (OPEN) for zinc finger target site sequences
por: Sander, Jeffry D, et al.
Publicado: (2010)

Avoiding false-positive signals with nuclease-vulnerable molecular beacons in single living cells
por: Chen, Antony K., et al.
Publicado: (2007)

High-efficiency and heritable gene targeting in mouse by transcription activator-like effector nucleases
por: Qiu, Zhongwei, et al.
Publicado: (2013)

Multiplexed and tunable transcriptional activation by promoter insertion using nuclease-assisted vector integration
por: Brown, Alexander, et al.
Publicado: (2019)

Comparing Zinc Finger Nucleases and Transcription Activator-Like Effector Nucleases for Gene Targeting in Drosophila
por: Beumer, Kelly J., et al.
Publicado: (2013)

Zinc Finger Database (ZiFDB): a repository for information on C2H2 zinc fingers and engineered zinc-finger arrays
por: Fu, Fengli, et al.
Publicado: (2009)

Targeted genome engineering via zinc finger nucleases
por: Kim, Seokjoong, et al.
Publicado: (2010)

Profiling the DNA-binding specificities of engineered Cys2His2 zinc finger domains using a rapid cell-based method
por: Meng, Xiangdong, et al.
Publicado: (2007)

Cannot write session to /tmp/vufind_sessions/sess_06ch694n1u28gv4jjj05cb7q33