Directed Evolution of Aminoglycoside Phosphotransferase (3′) Type IIIa Variants That Inactivate Amikacin but Impose Significant Fitness Costs

The rules that govern adaptive protein evolution remain incompletely understood. Aminoglycoside aminotransferase (3′) type IIIa (hereafter abbreviated APH(3′)-IIIa) is a good model enzyme because it inactivates kanamycin efficiently; it recognizes other aminoglycoside antibiotics, including amikacin...

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Detalles Bibliográficos
Autores principales: Kramer, Joseph R., Matsumura, Ichiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3800079/
https://www.ncbi.nlm.nih.gov/pubmed/24204655
http://dx.doi.org/10.1371/journal.pone.0076687

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3800079/
https://www.ncbi.nlm.nih.gov/pubmed/24204655
http://dx.doi.org/10.1371/journal.pone.0076687

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