Murine hepatitis virus nsp4 N258T mutants are not temperature-sensitive

Coronavirus replicase nsp4 is critical for virus-induced membrane modifications. An nsp4 mutant (N258T) of murine hepatitis virus (MHV) has been reported to be temperature-sensitive (ts) and to alter membrane targeting. We engineered and recovered all four possible codon variants of N258T in the clo...

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Detalles Bibliográficos
Autores principales: Beachboard, Dia C., Lu, Xiaotao, Baker, Susan C., Denison, Mark R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2013
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3804408/
https://www.ncbi.nlm.nih.gov/pubmed/23099203
http://dx.doi.org/10.1016/j.virol.2012.10.001
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author Beachboard, Dia C.
Lu, Xiaotao
Baker, Susan C.
Denison, Mark R.
author_facet Beachboard, Dia C.
Lu, Xiaotao
Baker, Susan C.
Denison, Mark R.
author_sort Beachboard, Dia C.
collection PubMed
description Coronavirus replicase nsp4 is critical for virus-induced membrane modifications. An nsp4 mutant (N258T) of murine hepatitis virus (MHV) has been reported to be temperature-sensitive (ts) and to alter membrane targeting. We engineered and recovered all four possible codon variants of N258T in the cloned MHV-A59 background. All mutant viruses demonstrated impaired replication compared to wildtype MHV, but no nsp4 N258T mutant virus was ts, and all variants colocalized with viral protein markers for replication complexes, but not with markers for mitochondria. This study emphasizes that complete genome sequencing may be necessary, even with directed and confirmed reverse genetic mutants.
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spelling pubmed-38044082014-01-20 Murine hepatitis virus nsp4 N258T mutants are not temperature-sensitive Beachboard, Dia C. Lu, Xiaotao Baker, Susan C. Denison, Mark R. Virology Rapid Communication Coronavirus replicase nsp4 is critical for virus-induced membrane modifications. An nsp4 mutant (N258T) of murine hepatitis virus (MHV) has been reported to be temperature-sensitive (ts) and to alter membrane targeting. We engineered and recovered all four possible codon variants of N258T in the cloned MHV-A59 background. All mutant viruses demonstrated impaired replication compared to wildtype MHV, but no nsp4 N258T mutant virus was ts, and all variants colocalized with viral protein markers for replication complexes, but not with markers for mitochondria. This study emphasizes that complete genome sequencing may be necessary, even with directed and confirmed reverse genetic mutants. Elsevier Inc. 2013-01-20 2012-10-23 /pmc/articles/PMC3804408/ /pubmed/23099203 http://dx.doi.org/10.1016/j.virol.2012.10.001 Text en Copyright © 2012 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Rapid Communication
Beachboard, Dia C.
Lu, Xiaotao
Baker, Susan C.
Denison, Mark R.
Murine hepatitis virus nsp4 N258T mutants are not temperature-sensitive
title Murine hepatitis virus nsp4 N258T mutants are not temperature-sensitive
title_full Murine hepatitis virus nsp4 N258T mutants are not temperature-sensitive
title_fullStr Murine hepatitis virus nsp4 N258T mutants are not temperature-sensitive
title_full_unstemmed Murine hepatitis virus nsp4 N258T mutants are not temperature-sensitive
title_short Murine hepatitis virus nsp4 N258T mutants are not temperature-sensitive
title_sort murine hepatitis virus nsp4 n258t mutants are not temperature-sensitive
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3804408/
https://www.ncbi.nlm.nih.gov/pubmed/23099203
http://dx.doi.org/10.1016/j.virol.2012.10.001
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