Global profiling of stimulus-induced polyadenylation in cells using a poly(A) trap

Polyadenylation of mRNA leads to increased protein expression in response to diverse stimuli, but it is difficult to identify mRNAs that become polyadenylated in living cells. Here we describe a click chemistry-compatible nucleoside analog that is selectively incorporated into poly(A) tails of trans...

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Detalles Bibliográficos
Autores principales: Curanovic, Dusica, Cohen, Michael, Singh, Irtisha, Slagle, Christopher E., Leslie, Christina S., Jaffrey, Samie R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3805764/
https://www.ncbi.nlm.nih.gov/pubmed/23995769
http://dx.doi.org/10.1038/nchembio.1334
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author Curanovic, Dusica
Cohen, Michael
Singh, Irtisha
Slagle, Christopher E.
Leslie, Christina S.
Jaffrey, Samie R.
author_facet Curanovic, Dusica
Cohen, Michael
Singh, Irtisha
Slagle, Christopher E.
Leslie, Christina S.
Jaffrey, Samie R.
author_sort Curanovic, Dusica
collection PubMed
description Polyadenylation of mRNA leads to increased protein expression in response to diverse stimuli, but it is difficult to identify mRNAs that become polyadenylated in living cells. Here we describe a click chemistry-compatible nucleoside analog that is selectively incorporated into poly(A) tails of transcripts in cells. Next-generation sequencing of labeled mRNAs enables a transcriptome-wide profile of polyadenylation and provides insights into the mRNA sequence elements that are correlated with polyadenylation.
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spelling pubmed-38057642014-05-01 Global profiling of stimulus-induced polyadenylation in cells using a poly(A) trap Curanovic, Dusica Cohen, Michael Singh, Irtisha Slagle, Christopher E. Leslie, Christina S. Jaffrey, Samie R. Nat Chem Biol Article Polyadenylation of mRNA leads to increased protein expression in response to diverse stimuli, but it is difficult to identify mRNAs that become polyadenylated in living cells. Here we describe a click chemistry-compatible nucleoside analog that is selectively incorporated into poly(A) tails of transcripts in cells. Next-generation sequencing of labeled mRNAs enables a transcriptome-wide profile of polyadenylation and provides insights into the mRNA sequence elements that are correlated with polyadenylation. 2013-09-01 2013-11 /pmc/articles/PMC3805764/ /pubmed/23995769 http://dx.doi.org/10.1038/nchembio.1334 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Curanovic, Dusica
Cohen, Michael
Singh, Irtisha
Slagle, Christopher E.
Leslie, Christina S.
Jaffrey, Samie R.
Global profiling of stimulus-induced polyadenylation in cells using a poly(A) trap
title Global profiling of stimulus-induced polyadenylation in cells using a poly(A) trap
title_full Global profiling of stimulus-induced polyadenylation in cells using a poly(A) trap
title_fullStr Global profiling of stimulus-induced polyadenylation in cells using a poly(A) trap
title_full_unstemmed Global profiling of stimulus-induced polyadenylation in cells using a poly(A) trap
title_short Global profiling of stimulus-induced polyadenylation in cells using a poly(A) trap
title_sort global profiling of stimulus-induced polyadenylation in cells using a poly(a) trap
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3805764/
https://www.ncbi.nlm.nih.gov/pubmed/23995769
http://dx.doi.org/10.1038/nchembio.1334
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