Effects of 2′-O-Methyl Nucleotide Substitution on EcoRI Endonuclease Cleavage Activities

To investigate the effect of sugar pucker conformation on DNA-protein interactions, we used 2′-O-methyl nucleotide (2′-OMeN) to modify the EcoRI recognition sequence -TGAATTCT-, and monitored the enzymatic cleavage process using FRET method. The 2′-O-methyl nucleotide has a C3′-endo sugar pucker con...

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Detalles Bibliográficos
Autores principales: Zhao, Guojie, Zhao, Bin, Tong, Zhaoxue, Mu, Runqing, Guan, Yifu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3806743/
https://www.ncbi.nlm.nih.gov/pubmed/24194862
http://dx.doi.org/10.1371/journal.pone.0077111

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3806743/
https://www.ncbi.nlm.nih.gov/pubmed/24194862
http://dx.doi.org/10.1371/journal.pone.0077111

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