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The Critical Role of DNA Extraction for Detection of Mycobacteria in Tissues

BACKGROUND: Nucleic acid-based methods offer promise for both targeted and exploratory investigations of microbes in tissue samples. As the starting material for such studies is a mixture of host and microbial DNA, we have critically evaluated the DNA extraction step to determine the quantitative an...

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Detalles Bibliográficos
Autores principales: Radomski, Nicolas, Kreitmann, Louis, McIntosh, Fiona, Behr, Marcel A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3806855/
https://www.ncbi.nlm.nih.gov/pubmed/24194951
http://dx.doi.org/10.1371/journal.pone.0078749