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Snap-, CLIP- and Halo-Tag Labelling of Budding Yeast Cells

Fluorescence microscopy of the localization and the spatial and temporal dynamics of specifically labelled proteins is an indispensable tool in cell biology. Besides fluorescent proteins as tags, tag-mediated labelling utilizing self-labelling proteins as the SNAP-, CLIP-, or the Halo-tag are widely...

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Detalles Bibliográficos
Autores principales: Stagge, Franziska, Mitronova, Gyuzel Y., Belov, Vladimir N., Wurm, Christian A., Jakobs, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3808294/
https://www.ncbi.nlm.nih.gov/pubmed/24205303
http://dx.doi.org/10.1371/journal.pone.0078745