A Guide RNA Sequence Design Platform for the CRISPR/Cas9 System for Model Organism Genomes

Cas9/CRISPR has been reported to efficiently induce targeted gene disruption and homologous recombination in both prokaryotic and eukaryotic cells. Thus, we developed a Guide RNA Sequence Design Platform for the Cas9/CRISPR silencing system for model organisms. The platform is easy to use for gRNA d...

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Detalles Bibliográficos
Autores principales: Ma, Ming, Ye, Adam Y., Zheng, Weiguo, Kong, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3809372/
https://www.ncbi.nlm.nih.gov/pubmed/24199189
http://dx.doi.org/10.1155/2013/270805
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author Ma, Ming
Ye, Adam Y.
Zheng, Weiguo
Kong, Lei
author_facet Ma, Ming
Ye, Adam Y.
Zheng, Weiguo
Kong, Lei
author_sort Ma, Ming
collection PubMed
description Cas9/CRISPR has been reported to efficiently induce targeted gene disruption and homologous recombination in both prokaryotic and eukaryotic cells. Thus, we developed a Guide RNA Sequence Design Platform for the Cas9/CRISPR silencing system for model organisms. The platform is easy to use for gRNA design with input query sequences. It finds potential targets by PAM and ranks them according to factors including uniqueness, SNP, RNA secondary structure, and AT content. The platform allows users to upload and share their experimental results. In addition, most guide RNA sequences from published papers have been put into our database.
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spelling pubmed-38093722013-11-06 A Guide RNA Sequence Design Platform for the CRISPR/Cas9 System for Model Organism Genomes Ma, Ming Ye, Adam Y. Zheng, Weiguo Kong, Lei Biomed Res Int Research Article Cas9/CRISPR has been reported to efficiently induce targeted gene disruption and homologous recombination in both prokaryotic and eukaryotic cells. Thus, we developed a Guide RNA Sequence Design Platform for the Cas9/CRISPR silencing system for model organisms. The platform is easy to use for gRNA design with input query sequences. It finds potential targets by PAM and ranks them according to factors including uniqueness, SNP, RNA secondary structure, and AT content. The platform allows users to upload and share their experimental results. In addition, most guide RNA sequences from published papers have been put into our database. Hindawi Publishing Corporation 2013 2013-10-03 /pmc/articles/PMC3809372/ /pubmed/24199189 http://dx.doi.org/10.1155/2013/270805 Text en Copyright © 2013 Ming Ma et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ma, Ming
Ye, Adam Y.
Zheng, Weiguo
Kong, Lei
A Guide RNA Sequence Design Platform for the CRISPR/Cas9 System for Model Organism Genomes
title A Guide RNA Sequence Design Platform for the CRISPR/Cas9 System for Model Organism Genomes
title_full A Guide RNA Sequence Design Platform for the CRISPR/Cas9 System for Model Organism Genomes
title_fullStr A Guide RNA Sequence Design Platform for the CRISPR/Cas9 System for Model Organism Genomes
title_full_unstemmed A Guide RNA Sequence Design Platform for the CRISPR/Cas9 System for Model Organism Genomes
title_short A Guide RNA Sequence Design Platform for the CRISPR/Cas9 System for Model Organism Genomes
title_sort guide rna sequence design platform for the crispr/cas9 system for model organism genomes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3809372/
https://www.ncbi.nlm.nih.gov/pubmed/24199189
http://dx.doi.org/10.1155/2013/270805
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