Development and Validation of an ELISA at Acidic pH for the Quantitative Determination of IL-13 in Human Plasma and Serum

A novel sandwich ELISA for the quantitative and sensitive determination of IL-13 in human serum and plasma was established. The assay employs an incubation step at acidic pH, which was shown to decrease nonspecific binding and interference from IL-13 binding proteins. The assay was validated and was...

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Detalles Bibliográficos
Autores principales: Doucet, Julie, Zhao, An, Fu, Jean, Avrameas, Alexandre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3810116/
https://www.ncbi.nlm.nih.gov/pubmed/24222716
http://dx.doi.org/10.1155/2013/290670

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3810116/
https://www.ncbi.nlm.nih.gov/pubmed/24222716
http://dx.doi.org/10.1155/2013/290670

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