Development and Validation of an ELISA at Acidic pH for the Quantitative Determination of IL-13 in Human Plasma and Serum

A novel sandwich ELISA for the quantitative and sensitive determination of IL-13 in human serum and plasma was established. The assay employs an incubation step at acidic pH, which was shown to decrease nonspecific binding and interference from IL-13 binding proteins. The assay was validated and was...

Descripción completa

Detalles Bibliográficos
Autores principales: Doucet, Julie, Zhao, An, Fu, Jean, Avrameas, Alexandre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3810116/
https://www.ncbi.nlm.nih.gov/pubmed/24222716
http://dx.doi.org/10.1155/2013/290670
_version_ 1782288748244893696
author Doucet, Julie
Zhao, An
Fu, Jean
Avrameas, Alexandre
author_facet Doucet, Julie
Zhao, An
Fu, Jean
Avrameas, Alexandre
author_sort Doucet, Julie
collection PubMed
description A novel sandwich ELISA for the quantitative and sensitive determination of IL-13 in human serum and plasma was established. The assay employs an incubation step at acidic pH, which was shown to decrease nonspecific binding and interference from IL-13 binding proteins. The assay was validated and was shown to be accurate and precise over the entire quantification range (0.59 to 68.4 pg/mL in human EDTA plasma). The validated assay was successfully applied to samples from healthy volunteers and patients with atopic seasonal rhinitis. The assay is suitable for use in clinical trials to monitor efficacy or pharmacodynamic effects of drug candidates.
format Online
Article
Text
id pubmed-3810116
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-38101162013-11-11 Development and Validation of an ELISA at Acidic pH for the Quantitative Determination of IL-13 in Human Plasma and Serum Doucet, Julie Zhao, An Fu, Jean Avrameas, Alexandre Dis Markers Research Article A novel sandwich ELISA for the quantitative and sensitive determination of IL-13 in human serum and plasma was established. The assay employs an incubation step at acidic pH, which was shown to decrease nonspecific binding and interference from IL-13 binding proteins. The assay was validated and was shown to be accurate and precise over the entire quantification range (0.59 to 68.4 pg/mL in human EDTA plasma). The validated assay was successfully applied to samples from healthy volunteers and patients with atopic seasonal rhinitis. The assay is suitable for use in clinical trials to monitor efficacy or pharmacodynamic effects of drug candidates. Hindawi Publishing Corporation 2013 2013-10-02 /pmc/articles/PMC3810116/ /pubmed/24222716 http://dx.doi.org/10.1155/2013/290670 Text en Copyright © 2013 Julie Doucet et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Doucet, Julie
Zhao, An
Fu, Jean
Avrameas, Alexandre
Development and Validation of an ELISA at Acidic pH for the Quantitative Determination of IL-13 in Human Plasma and Serum
title Development and Validation of an ELISA at Acidic pH for the Quantitative Determination of IL-13 in Human Plasma and Serum
title_full Development and Validation of an ELISA at Acidic pH for the Quantitative Determination of IL-13 in Human Plasma and Serum
title_fullStr Development and Validation of an ELISA at Acidic pH for the Quantitative Determination of IL-13 in Human Plasma and Serum
title_full_unstemmed Development and Validation of an ELISA at Acidic pH for the Quantitative Determination of IL-13 in Human Plasma and Serum
title_short Development and Validation of an ELISA at Acidic pH for the Quantitative Determination of IL-13 in Human Plasma and Serum
title_sort development and validation of an elisa at acidic ph for the quantitative determination of il-13 in human plasma and serum
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3810116/
https://www.ncbi.nlm.nih.gov/pubmed/24222716
http://dx.doi.org/10.1155/2013/290670
work_keys_str_mv AT doucetjulie developmentandvalidationofanelisaatacidicphforthequantitativedeterminationofil13inhumanplasmaandserum
AT zhaoan developmentandvalidationofanelisaatacidicphforthequantitativedeterminationofil13inhumanplasmaandserum
AT fujean developmentandvalidationofanelisaatacidicphforthequantitativedeterminationofil13inhumanplasmaandserum
AT avrameasalexandre developmentandvalidationofanelisaatacidicphforthequantitativedeterminationofil13inhumanplasmaandserum

Ejemplares similares