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High-throughput instant quantification of protein expression and purity based on photoactive yellow protein turn off/on label
Quantifying the concentration and purity of a target protein is essential for high-throughput protein expression test and rapid screening of highly soluble proteins. However, conventional methods such as PAGE and dot blot assay generally involve multiple time-consuming tasks requiring hours or do no...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3810716/ https://www.ncbi.nlm.nih.gov/pubmed/23740751 http://dx.doi.org/10.1002/pro.2286 |