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High-throughput instant quantification of protein expression and purity based on photoactive yellow protein turn off/on label

Quantifying the concentration and purity of a target protein is essential for high-throughput protein expression test and rapid screening of highly soluble proteins. However, conventional methods such as PAGE and dot blot assay generally involve multiple time-consuming tasks requiring hours or do no...

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Detalles Bibliográficos
Autores principales: Kim, Youngmin, Ganesan, Prabhakar, Ihee, Hyotcherl
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3810716/
https://www.ncbi.nlm.nih.gov/pubmed/23740751
http://dx.doi.org/10.1002/pro.2286

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