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Random and direct mutagenesis to enhance protein secretion in Ashbya gossypii

To improve the general secretion ability of the biotechnologically relevant fungus Ashbya gossypii, random mutagenesis with ethyl methane sulfonate (EMS) was performed. The selection and screening strategy followed revealed mutants with improved secretion of heterologous Trichoderma reesei endogluca...

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Detalles Bibliográficos
Autores principales: Ribeiro, Orquídea, Magalhães, Frederico, Aguiar, Tatiana Q, Wiebe, Marilyn G, Penttilä, Merja, Domingues, Lucília
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Landes Bioscience 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813532/
https://www.ncbi.nlm.nih.gov/pubmed/23644277
http://dx.doi.org/10.4161/bioe.24653