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Random and direct mutagenesis to enhance protein secretion in Ashbya gossypii
To improve the general secretion ability of the biotechnologically relevant fungus Ashbya gossypii, random mutagenesis with ethyl methane sulfonate (EMS) was performed. The selection and screening strategy followed revealed mutants with improved secretion of heterologous Trichoderma reesei endogluca...
Autores principales: | Ribeiro, Orquídea, Magalhães, Frederico, Aguiar, Tatiana Q, Wiebe, Marilyn G, Penttilä, Merja, Domingues, Lucília |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Landes Bioscience
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813532/ https://www.ncbi.nlm.nih.gov/pubmed/23644277 http://dx.doi.org/10.4161/bioe.24653 |
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