Heritable Gene Knockout in Caenorhabditis elegans by Direct Injection of Cas9–sgRNA Ribonucleoproteins

We present a novel method of targeted gene disruption that involves direct injection of recombinant Cas9 protein complexed with guide RNA into the gonad of the nematode Caenorhabditis elegans. Biallelic mutants were recovered among the F(1) progeny, demonstrating the high efficiency of this method.

Detalles Bibliográficos
Autores principales: Cho, Seung Woo, Lee, Jihyun, Carroll, Dana, Kim, Jin-Soo, Lee, Junho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2013
Materias:
Notes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813847/
https://www.ncbi.nlm.nih.gov/pubmed/23979576
http://dx.doi.org/10.1534/genetics.113.155853

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813847/
https://www.ncbi.nlm.nih.gov/pubmed/23979576
http://dx.doi.org/10.1534/genetics.113.155853

Ejemplares similares