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Heritable Gene Knockout in Caenorhabditis elegans by Direct Injection of Cas9–sgRNA Ribonucleoproteins
We present a novel method of targeted gene disruption that involves direct injection of recombinant Cas9 protein complexed with guide RNA into the gonad of the nematode Caenorhabditis elegans. Biallelic mutants were recovered among the F(1) progeny, demonstrating the high efficiency of this method.
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Genetics Society of America
2013
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813847/ https://www.ncbi.nlm.nih.gov/pubmed/23979576 http://dx.doi.org/10.1534/genetics.113.155853 |
| _version_ | 1782289173651128320 |
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| author | Cho, Seung Woo Lee, Jihyun Carroll, Dana Kim, Jin-Soo Lee, Junho |
| author_facet | Cho, Seung Woo Lee, Jihyun Carroll, Dana Kim, Jin-Soo Lee, Junho |
| author_sort | Cho, Seung Woo |
| collection | PubMed |
| description | We present a novel method of targeted gene disruption that involves direct injection of recombinant Cas9 protein complexed with guide RNA into the gonad of the nematode Caenorhabditis elegans. Biallelic mutants were recovered among the F(1) progeny, demonstrating the high efficiency of this method. |
| format | Online Article Text |
| id | pubmed-3813847 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2013 |
| publisher | Genetics Society of America |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-38138472013-11-01 Heritable Gene Knockout in Caenorhabditis elegans by Direct Injection of Cas9–sgRNA Ribonucleoproteins Cho, Seung Woo Lee, Jihyun Carroll, Dana Kim, Jin-Soo Lee, Junho Genetics Notes We present a novel method of targeted gene disruption that involves direct injection of recombinant Cas9 protein complexed with guide RNA into the gonad of the nematode Caenorhabditis elegans. Biallelic mutants were recovered among the F(1) progeny, demonstrating the high efficiency of this method. Genetics Society of America 2013-11 /pmc/articles/PMC3813847/ /pubmed/23979576 http://dx.doi.org/10.1534/genetics.113.155853 Text en Copyright © 2013 by the Genetics Society of America Available freely online through the author-supported open access option. |
| spellingShingle | Notes Cho, Seung Woo Lee, Jihyun Carroll, Dana Kim, Jin-Soo Lee, Junho Heritable Gene Knockout in Caenorhabditis elegans by Direct Injection of Cas9–sgRNA Ribonucleoproteins |
| title | Heritable Gene Knockout in Caenorhabditis elegans by Direct Injection of Cas9–sgRNA Ribonucleoproteins |
| title_full | Heritable Gene Knockout in Caenorhabditis elegans by Direct Injection of Cas9–sgRNA Ribonucleoproteins |
| title_fullStr | Heritable Gene Knockout in Caenorhabditis elegans by Direct Injection of Cas9–sgRNA Ribonucleoproteins |
| title_full_unstemmed | Heritable Gene Knockout in Caenorhabditis elegans by Direct Injection of Cas9–sgRNA Ribonucleoproteins |
| title_short | Heritable Gene Knockout in Caenorhabditis elegans by Direct Injection of Cas9–sgRNA Ribonucleoproteins |
| title_sort | heritable gene knockout in caenorhabditis elegans by direct injection of cas9–sgrna ribonucleoproteins |
| topic | Notes |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813847/ https://www.ncbi.nlm.nih.gov/pubmed/23979576 http://dx.doi.org/10.1534/genetics.113.155853 |
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