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Robust and specific ratiometric biosensing using a copper-free clicked quantum dot–DNA aptamer sensor

We report herein the successful preparation of a compact and functional CdSe–ZnS core–shell quantum dot (QD)–DNA conjugate via highly efficient copper-free “click chemistry” (CFCC) between a dihydro-lipoic acid–polyethylene glycol–azide (DHLA–PEG–N(3)) capped QD and a cyclooctyne modified DNA. This...

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Autores principales: Zhang, Haiyan, Feng, Guoqiang, Guo, Yuan, Zhou, Dejian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3814187/
https://www.ncbi.nlm.nih.gov/pubmed/24056667
http://dx.doi.org/10.1039/c3nr02897f
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author Zhang, Haiyan
Feng, Guoqiang
Guo, Yuan
Zhou, Dejian
author_facet Zhang, Haiyan
Feng, Guoqiang
Guo, Yuan
Zhou, Dejian
author_sort Zhang, Haiyan
collection PubMed
description We report herein the successful preparation of a compact and functional CdSe–ZnS core–shell quantum dot (QD)–DNA conjugate via highly efficient copper-free “click chemistry” (CFCC) between a dihydro-lipoic acid–polyethylene glycol–azide (DHLA–PEG–N(3)) capped QD and a cyclooctyne modified DNA. This represents an excellent balance between the requirements of high sensitivity, robustness and specificity for the QD-FRET (Förster resonance energy transfer) based sensor as confirmed by a detailed FRET analysis on the QD–DNA conjugate, yielding a relatively short donor–acceptor distance of ∼5.8 nm. We show that this CFCC clicked QD–DNA conjugate is not only able to retain the native fluorescence quantum yield (QY) of the parent DHLA–PEG–N(3) capped QD, but also well-suited for robust and specific biosensing; it can directly quantitate, at the pM level, both labelled and unlabelled complementary DNA probes with a good SNP (single-nucleotide polymorphism) discrimination ability in complex media, e.g. 10% human serum via target-binding induced FRET changes between the QD donor and the dye acceptor. Furthermore, this sensor has also been successfully exploited for the detection, at the pM level, of a specific protein target (thrombin) via the encoded anti-thrombin aptamer sequence in the QD–DNA conjugate.
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spelling pubmed-38141872013-10-31 Robust and specific ratiometric biosensing using a copper-free clicked quantum dot–DNA aptamer sensor Zhang, Haiyan Feng, Guoqiang Guo, Yuan Zhou, Dejian Nanoscale Chemistry We report herein the successful preparation of a compact and functional CdSe–ZnS core–shell quantum dot (QD)–DNA conjugate via highly efficient copper-free “click chemistry” (CFCC) between a dihydro-lipoic acid–polyethylene glycol–azide (DHLA–PEG–N(3)) capped QD and a cyclooctyne modified DNA. This represents an excellent balance between the requirements of high sensitivity, robustness and specificity for the QD-FRET (Förster resonance energy transfer) based sensor as confirmed by a detailed FRET analysis on the QD–DNA conjugate, yielding a relatively short donor–acceptor distance of ∼5.8 nm. We show that this CFCC clicked QD–DNA conjugate is not only able to retain the native fluorescence quantum yield (QY) of the parent DHLA–PEG–N(3) capped QD, but also well-suited for robust and specific biosensing; it can directly quantitate, at the pM level, both labelled and unlabelled complementary DNA probes with a good SNP (single-nucleotide polymorphism) discrimination ability in complex media, e.g. 10% human serum via target-binding induced FRET changes between the QD donor and the dye acceptor. Furthermore, this sensor has also been successfully exploited for the detection, at the pM level, of a specific protein target (thrombin) via the encoded anti-thrombin aptamer sequence in the QD–DNA conjugate. Royal Society of Chemistry 2013-11-07 2013-09-11 /pmc/articles/PMC3814187/ /pubmed/24056667 http://dx.doi.org/10.1039/c3nr02897f Text en This journal is © The Royal Society of Chemistry 2013 http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Chemistry
Zhang, Haiyan
Feng, Guoqiang
Guo, Yuan
Zhou, Dejian
Robust and specific ratiometric biosensing using a copper-free clicked quantum dot–DNA aptamer sensor
title Robust and specific ratiometric biosensing using a copper-free clicked quantum dot–DNA aptamer sensor
title_full Robust and specific ratiometric biosensing using a copper-free clicked quantum dot–DNA aptamer sensor
title_fullStr Robust and specific ratiometric biosensing using a copper-free clicked quantum dot–DNA aptamer sensor
title_full_unstemmed Robust and specific ratiometric biosensing using a copper-free clicked quantum dot–DNA aptamer sensor
title_short Robust and specific ratiometric biosensing using a copper-free clicked quantum dot–DNA aptamer sensor
title_sort robust and specific ratiometric biosensing using a copper-free clicked quantum dot–dna aptamer sensor
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3814187/
https://www.ncbi.nlm.nih.gov/pubmed/24056667
http://dx.doi.org/10.1039/c3nr02897f
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