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Robust and specific ratiometric biosensing using a copper-free clicked quantum dot–DNA aptamer sensor
We report herein the successful preparation of a compact and functional CdSe–ZnS core–shell quantum dot (QD)–DNA conjugate via highly efficient copper-free “click chemistry” (CFCC) between a dihydro-lipoic acid–polyethylene glycol–azide (DHLA–PEG–N(3)) capped QD and a cyclooctyne modified DNA. This...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royal Society of Chemistry
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3814187/ https://www.ncbi.nlm.nih.gov/pubmed/24056667 http://dx.doi.org/10.1039/c3nr02897f |
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author | Zhang, Haiyan Feng, Guoqiang Guo, Yuan Zhou, Dejian |
author_facet | Zhang, Haiyan Feng, Guoqiang Guo, Yuan Zhou, Dejian |
author_sort | Zhang, Haiyan |
collection | PubMed |
description | We report herein the successful preparation of a compact and functional CdSe–ZnS core–shell quantum dot (QD)–DNA conjugate via highly efficient copper-free “click chemistry” (CFCC) between a dihydro-lipoic acid–polyethylene glycol–azide (DHLA–PEG–N(3)) capped QD and a cyclooctyne modified DNA. This represents an excellent balance between the requirements of high sensitivity, robustness and specificity for the QD-FRET (Förster resonance energy transfer) based sensor as confirmed by a detailed FRET analysis on the QD–DNA conjugate, yielding a relatively short donor–acceptor distance of ∼5.8 nm. We show that this CFCC clicked QD–DNA conjugate is not only able to retain the native fluorescence quantum yield (QY) of the parent DHLA–PEG–N(3) capped QD, but also well-suited for robust and specific biosensing; it can directly quantitate, at the pM level, both labelled and unlabelled complementary DNA probes with a good SNP (single-nucleotide polymorphism) discrimination ability in complex media, e.g. 10% human serum via target-binding induced FRET changes between the QD donor and the dye acceptor. Furthermore, this sensor has also been successfully exploited for the detection, at the pM level, of a specific protein target (thrombin) via the encoded anti-thrombin aptamer sequence in the QD–DNA conjugate. |
format | Online Article Text |
id | pubmed-3814187 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-38141872013-10-31 Robust and specific ratiometric biosensing using a copper-free clicked quantum dot–DNA aptamer sensor Zhang, Haiyan Feng, Guoqiang Guo, Yuan Zhou, Dejian Nanoscale Chemistry We report herein the successful preparation of a compact and functional CdSe–ZnS core–shell quantum dot (QD)–DNA conjugate via highly efficient copper-free “click chemistry” (CFCC) between a dihydro-lipoic acid–polyethylene glycol–azide (DHLA–PEG–N(3)) capped QD and a cyclooctyne modified DNA. This represents an excellent balance between the requirements of high sensitivity, robustness and specificity for the QD-FRET (Förster resonance energy transfer) based sensor as confirmed by a detailed FRET analysis on the QD–DNA conjugate, yielding a relatively short donor–acceptor distance of ∼5.8 nm. We show that this CFCC clicked QD–DNA conjugate is not only able to retain the native fluorescence quantum yield (QY) of the parent DHLA–PEG–N(3) capped QD, but also well-suited for robust and specific biosensing; it can directly quantitate, at the pM level, both labelled and unlabelled complementary DNA probes with a good SNP (single-nucleotide polymorphism) discrimination ability in complex media, e.g. 10% human serum via target-binding induced FRET changes between the QD donor and the dye acceptor. Furthermore, this sensor has also been successfully exploited for the detection, at the pM level, of a specific protein target (thrombin) via the encoded anti-thrombin aptamer sequence in the QD–DNA conjugate. Royal Society of Chemistry 2013-11-07 2013-09-11 /pmc/articles/PMC3814187/ /pubmed/24056667 http://dx.doi.org/10.1039/c3nr02897f Text en This journal is © The Royal Society of Chemistry 2013 http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Chemistry Zhang, Haiyan Feng, Guoqiang Guo, Yuan Zhou, Dejian Robust and specific ratiometric biosensing using a copper-free clicked quantum dot–DNA aptamer sensor |
title | Robust and specific ratiometric biosensing using a copper-free clicked quantum dot–DNA aptamer sensor
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title_full | Robust and specific ratiometric biosensing using a copper-free clicked quantum dot–DNA aptamer sensor
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title_fullStr | Robust and specific ratiometric biosensing using a copper-free clicked quantum dot–DNA aptamer sensor
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title_full_unstemmed | Robust and specific ratiometric biosensing using a copper-free clicked quantum dot–DNA aptamer sensor
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title_short | Robust and specific ratiometric biosensing using a copper-free clicked quantum dot–DNA aptamer sensor
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title_sort | robust and specific ratiometric biosensing using a copper-free clicked quantum dot–dna aptamer sensor |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3814187/ https://www.ncbi.nlm.nih.gov/pubmed/24056667 http://dx.doi.org/10.1039/c3nr02897f |
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