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Use of extremely short Förster resonance energy transfer probes in real-time polymerase chain reaction

Described in the article is a new approach for the sequence-specific detection of nucleic acids in real-time polymerase chain reaction (PCR) using fluorescently labeled oligonucleotide probes. The method is based on the production of PCR amplicons, which fold into dumbbell-like secondary structures...

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Detalles Bibliográficos
Autor principal: Kutyavin, Igor V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3814350/
https://www.ncbi.nlm.nih.gov/pubmed/24013564
http://dx.doi.org/10.1093/nar/gkt782