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Use of extremely short Förster resonance energy transfer probes in real-time polymerase chain reaction
Described in the article is a new approach for the sequence-specific detection of nucleic acids in real-time polymerase chain reaction (PCR) using fluorescently labeled oligonucleotide probes. The method is based on the production of PCR amplicons, which fold into dumbbell-like secondary structures...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Oxford University Press
2013
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3814350/ https://www.ncbi.nlm.nih.gov/pubmed/24013564 http://dx.doi.org/10.1093/nar/gkt782 |