Efficient generation of large-scale genome-modified mice using gRNA and CAS9 endonuclease

The generation of genome-modified animals is a powerful approach to analyze gene functions. The CAS9/guide RNA (gRNA) system is expected to become widely used for the efficient generation of genome-modified animals, but detailed studies on optimum conditions and availability are limited. In the pres...

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Detalles Bibliográficos
Autores principales: Fujii, Wataru, Kawasaki, Kurenai, Sugiura, Koji, Naito, Kunihiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3814358/
https://www.ncbi.nlm.nih.gov/pubmed/23997119
http://dx.doi.org/10.1093/nar/gkt772

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3814358/
https://www.ncbi.nlm.nih.gov/pubmed/23997119
http://dx.doi.org/10.1093/nar/gkt772

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