Directed evolution of Aspergillus niger glucoamylase to increase thermostability

Using directed evolution and site‐directed mutagenesis, we have isolated a highly thermostable variant of Aspergillus niger glucoamylase (GA), designated CR2‐1. CR2‐1 includes the previously described mutations Asn20Cys and Ala27Cys (forming a new disulfide bond), Ser30Pro, Thr62Ala, Ser119Pro, Gly1...

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Detalles Bibliográficos
Autores principales: McDaniel, Allison, Fuchs, Erica, Liu, Ying, Ford, Clark
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2008
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815294/
https://www.ncbi.nlm.nih.gov/pubmed/21261873
http://dx.doi.org/10.1111/j.1751-7915.2008.00055.x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815294/
https://www.ncbi.nlm.nih.gov/pubmed/21261873
http://dx.doi.org/10.1111/j.1751-7915.2008.00055.x

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