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Mining bacterial genomes for novel arylesterase activity

One hundred and seventy‐one genes encoding potential esterases from 11 bacterial genomes were cloned and overexpressed in Escherichia coli; 74 of the clones produced soluble proteins. All 74 soluble proteins were purified and screened for esterase activity; 36 proteins showed carboxyl esterase activ...

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Detalles Bibliográficos
Autores principales: Wang, Lijun, Mavisakalyan, Valentina, Tillier, Elisabeth R. M., Clark, Greg W., Savchenko, Alexei V., Yakunin, Alexander F., Master, Emma R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815341/
https://www.ncbi.nlm.nih.gov/pubmed/21255363
http://dx.doi.org/10.1111/j.1751-7915.2010.00185.x