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Overcoming the restriction barrier to plasmid transformation and targeted mutagenesis in Bifidobacterium breve UCC2003

In silico analysis of the Bifidobacterium breve UCC2003 genome predicted two distinct loci, which encode three different restriction/modification systems, each comprising a modification methylase and a restriction endonuclease. Based on sequence homology and observed protection against restriction w...

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Detalles Bibliográficos
Autores principales: O'Connell Motherway, Mary, O'Driscoll, Jonathan, Fitzgerald, Gerald F., Van Sinderen, Douwe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815753/
https://www.ncbi.nlm.nih.gov/pubmed/21261927
http://dx.doi.org/10.1111/j.1751-7915.2008.00071.x