The expression of recombinant genes in Escherichia coli can be strongly stimulated at the transcript production level by mutating the DNA‐region corresponding to the 5′‐untranslated part of mRNA

Secondary structures and the short Shine–Dalgarno sequence in the 5′‐untranslated region of bacterial mRNAs (UTR) are known to affect gene expression at the level of translation. Here we report the use of random combinatorial DNA sequence libraries to study UTR function, applying the strong, σ(32)/σ...

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Detalles Bibliográficos
Autores principales: Berg, Laila, Lale, Rahmi, Bakke, Ingrid, Burroughs, Nigel, Valla, Svein
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2009
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815758/
https://www.ncbi.nlm.nih.gov/pubmed/21261932
http://dx.doi.org/10.1111/j.1751-7915.2009.00107.x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815758/
https://www.ncbi.nlm.nih.gov/pubmed/21261932
http://dx.doi.org/10.1111/j.1751-7915.2009.00107.x

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