Cargando…
Simple, fast and high‐efficiency transformation system for directed evolution of cellulase in Bacillus subtilis
Bacillus subtilis can serve as a powerful platform for directed evolution, especially for secretory enzymes. However, cloning and transformation of a DNA mutant library in B. subtilis are not as easy as they are in Escherichia coli. For direct transformation of B. subtilis, here we developed a new p...
| Autores principales: | , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Blackwell Publishing Ltd
2011
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815800/ https://www.ncbi.nlm.nih.gov/pubmed/21255377 http://dx.doi.org/10.1111/j.1751-7915.2010.00230.x |