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Directed evolution of the transcription factor XylS for development of improved expression systems

The inducible Pm promoter together with its cognate positive transcription regulator XylS has been shown to be useful for recombinant protein production under high cell density conditions. Here we report directed evolution of XylS resulting in mutant proteins with increased ability to stimulate tran...

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Detalles Bibliográficos
Autores principales: Vee Aune, Trond Erik, Bakke, Ingrid, Drabløs, Finn, Lale, Rahmi, Brautaset, Trygve, Valla, Svein
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815945/
https://www.ncbi.nlm.nih.gov/pubmed/21255304
http://dx.doi.org/10.1111/j.1751-7915.2009.00126.x