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Inter‐conversion of catalytic abilities in a bifunctional carboxyl/feruloyl‐esterase from earthworm gut metagenome

Carboxyl esterases (CE) exhibit various reaction specificities despite of their overall structural similarity. In present study we have exploited functional metagenomics, saturation mutagenesis and experimental protein evolution to explore residues that have a significant role in substrate discrimin...

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Detalles Bibliográficos
Autores principales: Vieites, José María, Ghazi, Azam, Beloqui, Ana, Polaina, Julio, Andreu, José M., Golyshina, Olga V., Nechitaylo, Taras Y., Waliczek, Agnes, Yakimov, Michail M., Golyshin, Peter N., Ferrer, Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815946/
https://www.ncbi.nlm.nih.gov/pubmed/21255305
http://dx.doi.org/10.1111/j.1751-7915.2009.00135.x