Development of a general methodology for labelling peptide–morpholino oligonucleotide conjugates using alkyne–azide click chemistry

We describe a general methodology for fluorescent labelling of peptide conjugates of phosphorodiamidate morpholino oligonucleotides (PMOs) by alkyne functionalization of peptides, subsequent conjugation to PMOs and labelling with a fluorescent compound (Cy5-azide). Two peptide–PMO (PPMO) examples ar...

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Detalles Bibliográficos
Autores principales: Shabanpoor, Fazel, Gait, Michael J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2013
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3816334/
https://www.ncbi.nlm.nih.gov/pubmed/24064913
http://dx.doi.org/10.1039/c3cc46067c
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author Shabanpoor, Fazel
Gait, Michael J.
author_facet Shabanpoor, Fazel
Gait, Michael J.
author_sort Shabanpoor, Fazel
collection PubMed
description We describe a general methodology for fluorescent labelling of peptide conjugates of phosphorodiamidate morpholino oligonucleotides (PMOs) by alkyne functionalization of peptides, subsequent conjugation to PMOs and labelling with a fluorescent compound (Cy5-azide). Two peptide–PMO (PPMO) examples are shown. No detrimental effect of such labelled PMOs was seen in a biological assay.
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spelling pubmed-38163342013-11-06 Development of a general methodology for labelling peptide–morpholino oligonucleotide conjugates using alkyne–azide click chemistry Shabanpoor, Fazel Gait, Michael J. Chem Commun (Camb) Chemistry We describe a general methodology for fluorescent labelling of peptide conjugates of phosphorodiamidate morpholino oligonucleotides (PMOs) by alkyne functionalization of peptides, subsequent conjugation to PMOs and labelling with a fluorescent compound (Cy5-azide). Two peptide–PMO (PPMO) examples are shown. No detrimental effect of such labelled PMOs was seen in a biological assay. Royal Society of Chemistry 2013-11-11 2013-09-25 /pmc/articles/PMC3816334/ /pubmed/24064913 http://dx.doi.org/10.1039/c3cc46067c Text en This journal is © The Royal Society of Chemistry 2013 http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Chemistry
Shabanpoor, Fazel
Gait, Michael J.
Development of a general methodology for labelling peptide–morpholino oligonucleotide conjugates using alkyne–azide click chemistry
title Development of a general methodology for labelling peptide–morpholino oligonucleotide conjugates using alkyne–azide click chemistry
title_full Development of a general methodology for labelling peptide–morpholino oligonucleotide conjugates using alkyne–azide click chemistry
title_fullStr Development of a general methodology for labelling peptide–morpholino oligonucleotide conjugates using alkyne–azide click chemistry
title_full_unstemmed Development of a general methodology for labelling peptide–morpholino oligonucleotide conjugates using alkyne–azide click chemistry
title_short Development of a general methodology for labelling peptide–morpholino oligonucleotide conjugates using alkyne–azide click chemistry
title_sort development of a general methodology for labelling peptide–morpholino oligonucleotide conjugates using alkyne–azide click chemistry
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3816334/
https://www.ncbi.nlm.nih.gov/pubmed/24064913
http://dx.doi.org/10.1039/c3cc46067c
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