Simplified Large-Scale Refolding, Purification, and Characterization of Recombinant Human Granulocyte-Colony Stimulating Factor in Escherichia coli

Granulocyte-colony stimulating factor (G-CSF) is a pleiotropic cytokine that stimulates the development of committed hematopoietic progenitor cells and enhances the functional activity of mature cells. Here, we report a simplified method for fed-batch culture as well as the purification of recombina...

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Detalles Bibliográficos
Autores principales: Kim, Chang Kyu, Lee, Chi Ho, Lee, Seung-Bae, Oh, Jae-Wook
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3817114/
https://www.ncbi.nlm.nih.gov/pubmed/24224041
http://dx.doi.org/10.1371/journal.pone.0080109

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3817114/
https://www.ncbi.nlm.nih.gov/pubmed/24224041
http://dx.doi.org/10.1371/journal.pone.0080109

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