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Simplified Large-Scale Refolding, Purification, and Characterization of Recombinant Human Granulocyte-Colony Stimulating Factor in Escherichia coli

Granulocyte-colony stimulating factor (G-CSF) is a pleiotropic cytokine that stimulates the development of committed hematopoietic progenitor cells and enhances the functional activity of mature cells. Here, we report a simplified method for fed-batch culture as well as the purification of recombina...

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Detalles Bibliográficos
Autores principales:	Kim, Chang Kyu, Lee, Chi Ho, Lee, Seung-Bae, Oh, Jae-Wook
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2013
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3817114/ https://www.ncbi.nlm.nih.gov/pubmed/24224041 http://dx.doi.org/10.1371/journal.pone.0080109

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