Combinatorial Engineering of 1-Deoxy-D-Xylulose 5-Phosphate Pathway Using Cross-Lapping In Vitro Assembly (CLIVA) Method

The ability to assemble multiple fragments of DNA into a plasmid in a single step is invaluable to studies in metabolic engineering and synthetic biology. Using phosphorothioate chemistry for high efficiency and site specific cleavage of sequences, a novel ligase independent cloning method (cross-la...

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Detalles Bibliográficos
Autores principales: Zou, Ruiyang, Zhou, Kang, Stephanopoulos, Gregory, Too, Heng Phon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3818232/
https://www.ncbi.nlm.nih.gov/pubmed/24223968
http://dx.doi.org/10.1371/journal.pone.0079557

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3818232/
https://www.ncbi.nlm.nih.gov/pubmed/24223968
http://dx.doi.org/10.1371/journal.pone.0079557

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