Evaluation of DNA extraction from granulocytes discarded in the separation medium after isolation of peripheral blood mononuclear cells and plasma from whole blood

BACKGROUND: Whole blood is generally processed for plasma and peripheral blood mononuclear cells (PBMCs) from granulocytes/erythrocytes using gradient centrifugation of blood with Histopaue-Ficoll. After separation of plasma and PBMCs, the residual erythrocytes/granulocytes, a rich source of DNA, is...

Descripción completa

Detalles Bibliográficos
Autores principales: Murray, Janná R, Rajeevan, Mangalathu S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3818442/
https://www.ncbi.nlm.nih.gov/pubmed/24176175
http://dx.doi.org/10.1186/1756-0500-6-440

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3818442/
https://www.ncbi.nlm.nih.gov/pubmed/24176175
http://dx.doi.org/10.1186/1756-0500-6-440

Ejemplares similares