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Rapid Assembly of Customized TALENs into Multiple Delivery Systems

Transcriptional activator-like effector nucleases (TALENs) have become a powerful tool for genome editing. Here we present an efficient TALEN assembly approach in which TALENs are assembled by direct Golden Gate ligation into Gateway(®) Entry vectors from a repeat variable di-residue (RVD) plasmid a...

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Detalles Bibliográficos
Autores principales: Zhang, Zhengxing, Zhang, Siliang, Huang, Xin, Orwig, Kyle E., Sheng, Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3820630/
https://www.ncbi.nlm.nih.gov/pubmed/24244669
http://dx.doi.org/10.1371/journal.pone.0080281