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Position of UNC-13 in the active zone regulates synaptic vesicle release probability and release kinetics

The presynaptic active zone proteins UNC-13/Munc13s are essential for synaptic vesicle (SV) exocytosis by directly interacting with SV fusion apparatus. An open question is how their association with active zones, hence their position to Ca(2+) entry sites, regulates SV release. The N-termini of maj...

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Autores principales: Zhou, Keming, Stawicki, Tamara M, Goncharov, Alexandr, Jin, Yishi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3821175/
https://www.ncbi.nlm.nih.gov/pubmed/24220508
http://dx.doi.org/10.7554/eLife.01180
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author Zhou, Keming
Stawicki, Tamara M
Goncharov, Alexandr
Jin, Yishi
author_facet Zhou, Keming
Stawicki, Tamara M
Goncharov, Alexandr
Jin, Yishi
author_sort Zhou, Keming
collection PubMed
description The presynaptic active zone proteins UNC-13/Munc13s are essential for synaptic vesicle (SV) exocytosis by directly interacting with SV fusion apparatus. An open question is how their association with active zones, hence their position to Ca(2+) entry sites, regulates SV release. The N-termini of major UNC-13/Munc13 isoforms contain a non-calcium binding C(2)A domain that mediates protein homo- or hetero-meric interactions. Here, we show that the C(2)A domain of Caenorhabditis elegans UNC-13 regulates release probability of evoked release and its precise active zone localization. Kinetics analysis of SV release supports that the proximity of UNC-13 to Ca(2+) entry sites, mediated by the C(2)A-domain containing N-terminus, is critical for accelerating neurotransmitter release. Additionally, the C(2)A domain is specifically required for spontaneous release. These data reveal multiple roles of UNC-13 C(2)A domain, and suggest that spontaneous release and the fast phase of evoked release may involve a common pool of SVs at the active zone. DOI: http://dx.doi.org/10.7554/eLife.01180.001
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spelling pubmed-38211752013-11-13 Position of UNC-13 in the active zone regulates synaptic vesicle release probability and release kinetics Zhou, Keming Stawicki, Tamara M Goncharov, Alexandr Jin, Yishi eLife Neuroscience The presynaptic active zone proteins UNC-13/Munc13s are essential for synaptic vesicle (SV) exocytosis by directly interacting with SV fusion apparatus. An open question is how their association with active zones, hence their position to Ca(2+) entry sites, regulates SV release. The N-termini of major UNC-13/Munc13 isoforms contain a non-calcium binding C(2)A domain that mediates protein homo- or hetero-meric interactions. Here, we show that the C(2)A domain of Caenorhabditis elegans UNC-13 regulates release probability of evoked release and its precise active zone localization. Kinetics analysis of SV release supports that the proximity of UNC-13 to Ca(2+) entry sites, mediated by the C(2)A-domain containing N-terminus, is critical for accelerating neurotransmitter release. Additionally, the C(2)A domain is specifically required for spontaneous release. These data reveal multiple roles of UNC-13 C(2)A domain, and suggest that spontaneous release and the fast phase of evoked release may involve a common pool of SVs at the active zone. DOI: http://dx.doi.org/10.7554/eLife.01180.001 eLife Sciences Publications, Ltd 2013-11-12 /pmc/articles/PMC3821175/ /pubmed/24220508 http://dx.doi.org/10.7554/eLife.01180 Text en Copyright © 2013, Zhou et al http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Zhou, Keming
Stawicki, Tamara M
Goncharov, Alexandr
Jin, Yishi
Position of UNC-13 in the active zone regulates synaptic vesicle release probability and release kinetics
title Position of UNC-13 in the active zone regulates synaptic vesicle release probability and release kinetics
title_full Position of UNC-13 in the active zone regulates synaptic vesicle release probability and release kinetics
title_fullStr Position of UNC-13 in the active zone regulates synaptic vesicle release probability and release kinetics
title_full_unstemmed Position of UNC-13 in the active zone regulates synaptic vesicle release probability and release kinetics
title_short Position of UNC-13 in the active zone regulates synaptic vesicle release probability and release kinetics
title_sort position of unc-13 in the active zone regulates synaptic vesicle release probability and release kinetics
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3821175/
https://www.ncbi.nlm.nih.gov/pubmed/24220508
http://dx.doi.org/10.7554/eLife.01180
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