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Development of Microsatellite Markers in Pungtungia herzi Using Next-Generation Sequencing and Cross-Species Amplification in the Genus Pseudopungtungia
Nuclear microsatellite markers for Pungtungia herzi were developed using a combination of next-generation sequencing and Sanger sequencing. One hundred primer sets in the flanking region of dinucleotide and trinucleotide repeat motifs were designed and tested for efficiency in polymerase chain react...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3821594/ https://www.ncbi.nlm.nih.gov/pubmed/24084733 http://dx.doi.org/10.3390/ijms141019923 |