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Conditional knockdown of hMRS2 results in loss of mitochondrial Mg (+) uptake and cell death
The human gene MRS2L encodes a mitochondrial protein distantly related to CorA Mg(2+) transport proteins. Constitutive shRNA-mediated knockdown of hMRS2 in human HEK-293 cell line was found here to cause death. To further study its role in Mg(2+) transport, we have established stable cell lines with...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3822876/ https://www.ncbi.nlm.nih.gov/pubmed/18384665 http://dx.doi.org/10.1111/j.1582-4934.2008.00328.x |
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author | Piskacek, Martin Zotova, Ludmila Zsurka, Gábor Schweyen, Rudolf J |
author_facet | Piskacek, Martin Zotova, Ludmila Zsurka, Gábor Schweyen, Rudolf J |
author_sort | Piskacek, Martin |
collection | PubMed |
description | The human gene MRS2L encodes a mitochondrial protein distantly related to CorA Mg(2+) transport proteins. Constitutive shRNA-mediated knockdown of hMRS2 in human HEK-293 cell line was found here to cause death. To further study its role in Mg(2+) transport, we have established stable cell lines with conditionally expressing shRNAs directed against hMRS2L. The cells expressing shRNA for several generations exhibited lower steady-state levels of free mitochondrial Mg(2+) ([Mg(2+)](m)) and reduced capacity of mitochondrial Mg(2+) uptake than control cells. Long-term expression of shRNAs resulted in loss of mitochondrial respiratory complex I, decreased mitochondrial membrane potential and cell death. We conclude that hMrs2 is the major transport protein for Mg (+) uptake into mitochondria and that expression of hMrs2 is essential for the maintenance of respiratory complex I and cell viability. |
format | Online Article Text |
id | pubmed-3822876 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-38228762015-04-27 Conditional knockdown of hMRS2 results in loss of mitochondrial Mg (+) uptake and cell death Piskacek, Martin Zotova, Ludmila Zsurka, Gábor Schweyen, Rudolf J J Cell Mol Med Articles The human gene MRS2L encodes a mitochondrial protein distantly related to CorA Mg(2+) transport proteins. Constitutive shRNA-mediated knockdown of hMRS2 in human HEK-293 cell line was found here to cause death. To further study its role in Mg(2+) transport, we have established stable cell lines with conditionally expressing shRNAs directed against hMRS2L. The cells expressing shRNA for several generations exhibited lower steady-state levels of free mitochondrial Mg(2+) ([Mg(2+)](m)) and reduced capacity of mitochondrial Mg(2+) uptake than control cells. Long-term expression of shRNAs resulted in loss of mitochondrial respiratory complex I, decreased mitochondrial membrane potential and cell death. We conclude that hMrs2 is the major transport protein for Mg (+) uptake into mitochondria and that expression of hMrs2 is essential for the maintenance of respiratory complex I and cell viability. Blackwell Publishing Ltd 2009-04 2008-03-31 /pmc/articles/PMC3822876/ /pubmed/18384665 http://dx.doi.org/10.1111/j.1582-4934.2008.00328.x Text en © 2009 The Authors Journal compilation © 2009 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd |
spellingShingle | Articles Piskacek, Martin Zotova, Ludmila Zsurka, Gábor Schweyen, Rudolf J Conditional knockdown of hMRS2 results in loss of mitochondrial Mg (+) uptake and cell death |
title | Conditional knockdown of hMRS2 results in loss of mitochondrial Mg (+) uptake and cell death |
title_full | Conditional knockdown of hMRS2 results in loss of mitochondrial Mg (+) uptake and cell death |
title_fullStr | Conditional knockdown of hMRS2 results in loss of mitochondrial Mg (+) uptake and cell death |
title_full_unstemmed | Conditional knockdown of hMRS2 results in loss of mitochondrial Mg (+) uptake and cell death |
title_short | Conditional knockdown of hMRS2 results in loss of mitochondrial Mg (+) uptake and cell death |
title_sort | conditional knockdown of hmrs2 results in loss of mitochondrial mg (+) uptake and cell death |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3822876/ https://www.ncbi.nlm.nih.gov/pubmed/18384665 http://dx.doi.org/10.1111/j.1582-4934.2008.00328.x |
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