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STAT1 deficiency in the heart protects against myocardial infarction by enhancing autophagy
Previous studies have shown that the transcription factor signal transducer and activator of transcription 1 (STAT1) activation is increased in primary cardiac myocytes exposed to simulated ischaemia/reperfusion injury. This promotes apoptotic cell death by enhancing the expression of pro-apoptotic...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3823301/ https://www.ncbi.nlm.nih.gov/pubmed/21447043 http://dx.doi.org/10.1111/j.1582-4934.2011.01323.x |
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author | McCormick, J Suleman, N Scarabelli, TM Knight, RA Latchman, DS Stephanou, A |
author_facet | McCormick, J Suleman, N Scarabelli, TM Knight, RA Latchman, DS Stephanou, A |
author_sort | McCormick, J |
collection | PubMed |
description | Previous studies have shown that the transcription factor signal transducer and activator of transcription 1 (STAT1) activation is increased in primary cardiac myocytes exposed to simulated ischaemia/reperfusion injury. This promotes apoptotic cell death by enhancing the expression of pro-apoptotic proteins. Autophagy has been demonstrated to play a cardioprotective role in the heart following myocardial infarction (MI). We therefore investigated the role of STAT1 in the intact heart subjected to MI and examined the contribution of autophagy in modulating the protective effect of STAT1 after MI injury. STAT1-deficient hearts had significantly smaller infarcts than wild-type hearts and this correlated with increased levels of autophagy shown by light chain 3 (LC3)-I/LC3-II conversion, and up-regulation of Atg12 and Beclin 1. Moreover, pre-treatment with the autophagy inhibitor 3-methyladenine reversed the cardioprotection observed in the STAT1-deficient hearts. These results reveal a new function of STAT1 in the control of autophagy and indicate a cross-talk between the cardioprotective versus the damaging effects of STAT1 in the intact heart exposed to MI injury. |
format | Online Article Text |
id | pubmed-3823301 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-38233012015-03-27 STAT1 deficiency in the heart protects against myocardial infarction by enhancing autophagy McCormick, J Suleman, N Scarabelli, TM Knight, RA Latchman, DS Stephanou, A J Cell Mol Med Original Articles Previous studies have shown that the transcription factor signal transducer and activator of transcription 1 (STAT1) activation is increased in primary cardiac myocytes exposed to simulated ischaemia/reperfusion injury. This promotes apoptotic cell death by enhancing the expression of pro-apoptotic proteins. Autophagy has been demonstrated to play a cardioprotective role in the heart following myocardial infarction (MI). We therefore investigated the role of STAT1 in the intact heart subjected to MI and examined the contribution of autophagy in modulating the protective effect of STAT1 after MI injury. STAT1-deficient hearts had significantly smaller infarcts than wild-type hearts and this correlated with increased levels of autophagy shown by light chain 3 (LC3)-I/LC3-II conversion, and up-regulation of Atg12 and Beclin 1. Moreover, pre-treatment with the autophagy inhibitor 3-methyladenine reversed the cardioprotection observed in the STAT1-deficient hearts. These results reveal a new function of STAT1 in the control of autophagy and indicate a cross-talk between the cardioprotective versus the damaging effects of STAT1 in the intact heart exposed to MI injury. Blackwell Publishing Ltd 2012-02 2012-01-27 /pmc/articles/PMC3823301/ /pubmed/21447043 http://dx.doi.org/10.1111/j.1582-4934.2011.01323.x Text en © 2011 The Authors Journal of Cellular and Molecular Medicine © 2011 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd |
spellingShingle | Original Articles McCormick, J Suleman, N Scarabelli, TM Knight, RA Latchman, DS Stephanou, A STAT1 deficiency in the heart protects against myocardial infarction by enhancing autophagy |
title | STAT1 deficiency in the heart protects against myocardial infarction by enhancing autophagy |
title_full | STAT1 deficiency in the heart protects against myocardial infarction by enhancing autophagy |
title_fullStr | STAT1 deficiency in the heart protects against myocardial infarction by enhancing autophagy |
title_full_unstemmed | STAT1 deficiency in the heart protects against myocardial infarction by enhancing autophagy |
title_short | STAT1 deficiency in the heart protects against myocardial infarction by enhancing autophagy |
title_sort | stat1 deficiency in the heart protects against myocardial infarction by enhancing autophagy |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3823301/ https://www.ncbi.nlm.nih.gov/pubmed/21447043 http://dx.doi.org/10.1111/j.1582-4934.2011.01323.x |
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