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Re-expression of alpha skeletal actin as a marker for dedifferentiation in cardiac pathologies

Differentiation of foetal cardiomyocytes is accompanied by sequential actin isoform expression, i.e. down-regulation of the ‘embryonic’ alpha smooth muscle actin, followed by an up-regulation of alpha skeletal actin (αSKA) and a final predominant expression of alpha cardiac actin (αCA). Our objectiv...

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Autores principales: Driesen, Ronald B, Verheyen, Fons K, Debie, Wiel, Blaauw, Erik, Babiker, Fawzi A, Cornelussen, Richard NM, Ausma, Jannie, Lenders, Marie-Hélène, Borgers, Marcel, Chaponnier, Christine, Ramaekers, Frans C S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3823406/
https://www.ncbi.nlm.nih.gov/pubmed/19538254
http://dx.doi.org/10.1111/j.1582-4934.2008.00523.x
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author Driesen, Ronald B
Verheyen, Fons K
Debie, Wiel
Blaauw, Erik
Babiker, Fawzi A
Cornelussen, Richard NM
Ausma, Jannie
Lenders, Marie-Hélène
Borgers, Marcel
Chaponnier, Christine
Ramaekers, Frans C S
author_facet Driesen, Ronald B
Verheyen, Fons K
Debie, Wiel
Blaauw, Erik
Babiker, Fawzi A
Cornelussen, Richard NM
Ausma, Jannie
Lenders, Marie-Hélène
Borgers, Marcel
Chaponnier, Christine
Ramaekers, Frans C S
author_sort Driesen, Ronald B
collection PubMed
description Differentiation of foetal cardiomyocytes is accompanied by sequential actin isoform expression, i.e. down-regulation of the ‘embryonic’ alpha smooth muscle actin, followed by an up-regulation of alpha skeletal actin (αSKA) and a final predominant expression of alpha cardiac actin (αCA). Our objective was to detect whether re-expression of αSKA occurred during cardiomyocyte dedifferentiation, a phenomenon that has been observed in different pathologies characterized by myocardial dysfunction. Immunohistochemistry of αCA, αSKA and cardiotin was performed on left ventricle biopsies from human patients after coronary bypass surgery. Furthermore, actin isoform expression was investigated in left ventricle samples of rabbit hearts suffering from pressure- and volume-overload and in adult rabbit ventricular cardiomyocytes during dedifferentiation in vitro. Atrial goat samples up to 16 weeks of sustained atrial fibrillation (AF) were studied ultrastructurally and were immunostained for αCA and αSKA. Up-regulation of αSKA was observed in human ventricular cardiomyocytes showing down-regulation of αCA and cardiotin. A patchy re-expression pattern of αSKA was observed in rabbit left ventricular tissue subjected to pressure- and volume-overload. Dedifferentiating cardiomyocytes in vitro revealed a degradation of the contractile apparatus and local re-expression of αSKA. Comparable αSKA staining patterns were found in several areas of atrial goat tissue during 16 weeks of AF together with a progressive glycogen accumulation at the same time intervals. The expression of αSKA in adult dedifferentiating cardiomyocytes, in combination with PAS-positive glycogen and decreased cardiotin expression, offers an additional tool in the evaluation of myocardial dysfunction and indicates major changes in the contractile properties of these cells.
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spelling pubmed-38234062015-04-27 Re-expression of alpha skeletal actin as a marker for dedifferentiation in cardiac pathologies Driesen, Ronald B Verheyen, Fons K Debie, Wiel Blaauw, Erik Babiker, Fawzi A Cornelussen, Richard NM Ausma, Jannie Lenders, Marie-Hélène Borgers, Marcel Chaponnier, Christine Ramaekers, Frans C S J Cell Mol Med Articles Differentiation of foetal cardiomyocytes is accompanied by sequential actin isoform expression, i.e. down-regulation of the ‘embryonic’ alpha smooth muscle actin, followed by an up-regulation of alpha skeletal actin (αSKA) and a final predominant expression of alpha cardiac actin (αCA). Our objective was to detect whether re-expression of αSKA occurred during cardiomyocyte dedifferentiation, a phenomenon that has been observed in different pathologies characterized by myocardial dysfunction. Immunohistochemistry of αCA, αSKA and cardiotin was performed on left ventricle biopsies from human patients after coronary bypass surgery. Furthermore, actin isoform expression was investigated in left ventricle samples of rabbit hearts suffering from pressure- and volume-overload and in adult rabbit ventricular cardiomyocytes during dedifferentiation in vitro. Atrial goat samples up to 16 weeks of sustained atrial fibrillation (AF) were studied ultrastructurally and were immunostained for αCA and αSKA. Up-regulation of αSKA was observed in human ventricular cardiomyocytes showing down-regulation of αCA and cardiotin. A patchy re-expression pattern of αSKA was observed in rabbit left ventricular tissue subjected to pressure- and volume-overload. Dedifferentiating cardiomyocytes in vitro revealed a degradation of the contractile apparatus and local re-expression of αSKA. Comparable αSKA staining patterns were found in several areas of atrial goat tissue during 16 weeks of AF together with a progressive glycogen accumulation at the same time intervals. The expression of αSKA in adult dedifferentiating cardiomyocytes, in combination with PAS-positive glycogen and decreased cardiotin expression, offers an additional tool in the evaluation of myocardial dysfunction and indicates major changes in the contractile properties of these cells. Blackwell Publishing Ltd 2009-05 2008-10-13 /pmc/articles/PMC3823406/ /pubmed/19538254 http://dx.doi.org/10.1111/j.1582-4934.2008.00523.x Text en © 2009 The Authors Journal compilation © 2009 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd
spellingShingle Articles
Driesen, Ronald B
Verheyen, Fons K
Debie, Wiel
Blaauw, Erik
Babiker, Fawzi A
Cornelussen, Richard NM
Ausma, Jannie
Lenders, Marie-Hélène
Borgers, Marcel
Chaponnier, Christine
Ramaekers, Frans C S
Re-expression of alpha skeletal actin as a marker for dedifferentiation in cardiac pathologies
title Re-expression of alpha skeletal actin as a marker for dedifferentiation in cardiac pathologies
title_full Re-expression of alpha skeletal actin as a marker for dedifferentiation in cardiac pathologies
title_fullStr Re-expression of alpha skeletal actin as a marker for dedifferentiation in cardiac pathologies
title_full_unstemmed Re-expression of alpha skeletal actin as a marker for dedifferentiation in cardiac pathologies
title_short Re-expression of alpha skeletal actin as a marker for dedifferentiation in cardiac pathologies
title_sort re-expression of alpha skeletal actin as a marker for dedifferentiation in cardiac pathologies
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3823406/
https://www.ncbi.nlm.nih.gov/pubmed/19538254
http://dx.doi.org/10.1111/j.1582-4934.2008.00523.x
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