Generating In Vivo Cloning Vectors for Parallel Cloning of Large Gene Clusters by Homologous Recombination

A robust method for the in vivo cloning of large gene clusters was developed based on homologous recombination (HR), requiring only the transformation of PCR products into Escherichia coli cells harboring a receiver plasmid. Positive clones were selected by an acquired antibiotic resistance, which w...

Descripción completa

Detalles Bibliográficos
Autores principales: Lee, Jeongmin, Rha, Eugene, Yeom, Soo-Jin, Lee, Dae-Hee, Choi, Eui-Sung, Lee, Seung-Goo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3823602/
https://www.ncbi.nlm.nih.gov/pubmed/24244585
http://dx.doi.org/10.1371/journal.pone.0079979

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3823602/
https://www.ncbi.nlm.nih.gov/pubmed/24244585
http://dx.doi.org/10.1371/journal.pone.0079979

Ejemplares similares