Simultaneous Expression of Displayed and Secreted Antibodies for Antibody Screen

The display of full-length antibody on the cell surface was achieved by fusing a transmembrane domain of the platelet-derived growth factor receptor (PDGFR) to the C-terminus of the heavy chain constant region. We also incorporated a furin cleavage site between the constant region and PDGFR transmem...

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Autores principales: Zhou, Yuanping, Wang, Junjie, Zhou, Ivan, Lou, Haibo, Li, Chang-Zheng, Chen, Zhen-Rui, Zhang, Zhe-Huan, Liu, Shuwen, Wu, Shuguang, Tan, Wanlong, Jiang, Shibo, Zhou, Chen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3823846/
https://www.ncbi.nlm.nih.gov/pubmed/24244593
http://dx.doi.org/10.1371/journal.pone.0080005
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author Zhou, Yuanping
Wang, Junjie
Zhou, Ivan
Lou, Haibo
Li, Chang-Zheng
Chen, Zhen-Rui
Zhang, Zhe-Huan
Liu, Shuwen
Wu, Shuguang
Tan, Wanlong
Jiang, Shibo
Zhou, Chen
author_facet Zhou, Yuanping
Wang, Junjie
Zhou, Ivan
Lou, Haibo
Li, Chang-Zheng
Chen, Zhen-Rui
Zhang, Zhe-Huan
Liu, Shuwen
Wu, Shuguang
Tan, Wanlong
Jiang, Shibo
Zhou, Chen
author_sort Zhou, Yuanping
collection PubMed
description The display of full-length antibody on the cell surface was achieved by fusing a transmembrane domain of the platelet-derived growth factor receptor (PDGFR) to the C-terminus of the heavy chain constant region. We also incorporated a furin cleavage site between the constant region and PDGFR transmembrane domain to obtain secreted antibodies. As a result, antibodies can be expressed simultaneously on the cell surface in a membrane-anchored version for screening and selecting through fluorescence-activated cell sorting (FACS) analysis, as well as in conditioned medium in a secreted version for function analysis.
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spelling pubmed-38238462013-11-15 Simultaneous Expression of Displayed and Secreted Antibodies for Antibody Screen Zhou, Yuanping Wang, Junjie Zhou, Ivan Lou, Haibo Li, Chang-Zheng Chen, Zhen-Rui Zhang, Zhe-Huan Liu, Shuwen Wu, Shuguang Tan, Wanlong Jiang, Shibo Zhou, Chen PLoS One Research Article The display of full-length antibody on the cell surface was achieved by fusing a transmembrane domain of the platelet-derived growth factor receptor (PDGFR) to the C-terminus of the heavy chain constant region. We also incorporated a furin cleavage site between the constant region and PDGFR transmembrane domain to obtain secreted antibodies. As a result, antibodies can be expressed simultaneously on the cell surface in a membrane-anchored version for screening and selecting through fluorescence-activated cell sorting (FACS) analysis, as well as in conditioned medium in a secreted version for function analysis. Public Library of Science 2013-11-11 /pmc/articles/PMC3823846/ /pubmed/24244593 http://dx.doi.org/10.1371/journal.pone.0080005 Text en © 2013 Zhou et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zhou, Yuanping
Wang, Junjie
Zhou, Ivan
Lou, Haibo
Li, Chang-Zheng
Chen, Zhen-Rui
Zhang, Zhe-Huan
Liu, Shuwen
Wu, Shuguang
Tan, Wanlong
Jiang, Shibo
Zhou, Chen
Simultaneous Expression of Displayed and Secreted Antibodies for Antibody Screen
title Simultaneous Expression of Displayed and Secreted Antibodies for Antibody Screen
title_full Simultaneous Expression of Displayed and Secreted Antibodies for Antibody Screen
title_fullStr Simultaneous Expression of Displayed and Secreted Antibodies for Antibody Screen
title_full_unstemmed Simultaneous Expression of Displayed and Secreted Antibodies for Antibody Screen
title_short Simultaneous Expression of Displayed and Secreted Antibodies for Antibody Screen
title_sort simultaneous expression of displayed and secreted antibodies for antibody screen
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3823846/
https://www.ncbi.nlm.nih.gov/pubmed/24244593
http://dx.doi.org/10.1371/journal.pone.0080005
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